Fabrication of bioinspired keratin/sodium alginate based biopolymeric mat loaded with herbal drug and green synthesized zinc oxide nanoparticles as a dual drug antimicrobial wound dressing.

Dual drug antibacterial wound dressings with biological materials possess crucial wound healing characteristics including biocompatibility, non-toxicity, degradability, mechanical strength and antibacterial properties. The study focusses on fabricating keratin (K)­sodium alginate (A) based wound dressings by loading green synthesized zinc oxide nanoparticles (ZnO NPs) using C. roseus (leaf extract) and M. recutita (Chamomile flower part) herbal drug (CH) as a bioactive dual antibacterial wound dressing for the first time. The optimized ZnO NPs and CH exhibits strong physiochemical and electrostatic interactions (FT-IR, XRD and SEM) on the fabricated K-A-CH-ZnO biopolymeric mats. Moreover, the tiny porous network of the biopolymeric mat enhances thermal stability, hydrophilicity, mechanical strength and explores the water vapor transmission (2538.07 g/m2/day) and oxygen permeability (7.38 ± 0.31 g/m2) to maintain moist environment and cell-material interactions. During enzymatic degradation studies, ZnO NPs and CH of biopolymeric mat not only retains structural integrity but also increases the characteristic of swelling with sustained drug release (57 %) in 144 h which accelerates wound healing process. Also, K-A-CH-ZnO mat exhibited excellent antibacterial effects against B. subtilis and E. coli. Furthermore, NIH 3T3 fibroblast cell behavior using MTT assay and in vivo evaluations of biopolymeric mat depicted enhanced biocompatibility with increased collagen deposition at the wound site as a prominent dual drug medicated antimicrobial wound dressing.

Development of chitosan-based cerium and titanium oxide loaded polycaprolactone for cutaneous wound healing and antibacterial applications.

Cerium dioxide (CeO2) based nanomaterials have emerged as promising dermal equivalents, promoting fibroblast infiltration and tissues regeneration. To enhance the antibacterial and wound healing activity, herein chitosan (CS)-CeO2 combined nano titanium dioxide (TiO2) complex loaded polycaprolactone (PCL) nanohybrid (CS-CeO2/TiO2/PCL) scaffolds were prepared through casting method. The nanohybrid scaffolds' physiochemical, morphological, mechanical, and biological properties were evaluated using advanced analytical techniques. Fourier transform infrared spectroscopy spectrum evidently depicted the various intermolecular interactions on the nanohybrid scaffolds. The developed scaffold exhibited the high swelling behavior and good degradability and permeability which is beneficial for absorbing wound transudation to fasten the healing efficacy. Moreover, CS-CeO2/TiO2/PCL scaffolds owned the better antibacterial activity against bacterial strains E. coli and S. aureus. Also, MTT assay on fibroblast (NIH 3T3) cells and immortalized human keratinocytes (HaCaT) cells indicated improved cell viability and proliferation. In vivo results revealed that the fabricated scaffold full aid to complete wound closure after 14 days which showed CS-CeO2/TiO2/PCL as the significant wound dressing material with potential antibacterial immunity.

Fabrication of dual layered biocompatible herbal biopatch from biological waste for skin - tissue regenerative applications.

A dual layered herbal biopolymeric patch (biopatch) with enhanced wound healing efficiency and skin mimicking functions was fabricated for skin-tissue regenerative applications. In this study, hoof keratin (KE) extracted from biological waste and gelatin (GE) was employed for KE-GE biosheet fabrication using a simple casting method. Further, the top layer of the fabricated KE-GE biosheet was coated with bioactive Matricaria recutita (Chamomile flower) extract (CH) with gelatin through an electrospraying method. The optimized dual layered herbal biopatch (KE-GE/GE-CH) exhibits strong physiochemical (FTIR, XRD TG-DTA), mechanical (tensile strength) and biological (in vitro and in vivo) studies. Moreover, the morphology (SEM) of soft mimetic biopatch possesses excellent cell-material interaction and cell proliferation which accelerates the wound healing process. Biopatch demonstrates a proven degradation profile with good swelling features to achieve more than 80% herbal drug release in 96 h. Antimicrobial properties also reveal the potential activity of biopatch against bacterial microbes. In addition, in vitro cell viability using NIH 3T3 fibroblast cell lines and in vivo investigations revealed that the biopatch is non-cytotoxic, increases collagen deposition and shows rapid reepithelialization at the wound site as a potential wound dressing. We anticipated that the biological hoof keratin and bioactive herbal extract coated biopatch could serve as a desirable wound dressing candidate to suit various skin tissue regenerative applications.